Sample Shipping Guideline
The conditions of the starting DNA are very important to achieve meaningful data. We promise to deliver the best possible outcome but at the same time, we request our clients to meet the minimum sample specification requirements as indicated below.
Quality and Quantity Specification
The ratio of OD260/280 is used as an indicator of sample purity where the values of 1.8–2.0 are an indication of a good DNA sample. However, this measurement can be compromised by the presence of RNA or small nucleic acid fragments such as nucleotides. Thus, gDNA samples should be carefully collected to ensure that they are free of any contaminants.
Gel electrophoresis is a powerful means for revealing the condition, including the actual presence or absence of DNA in a sample. Impurities, such as detergents or proteins, are typically revealed by the presence of smeared DNA bands. The interferences of RNA at 260 nm readings are often visible at the bottom of a gel. A ladder or smear below a band of interest may indicatethe nicking or other damage to the DNA. Where possible, a gel electrophoresis should be performed to assess the condition of the DNA sample.
The correct quantification of gDNA is essential in library construction. We recommend quantifying the starting genomic material by using a fluorescence‐based quantification method to accurately measure the quantity of dsDNA.
1. Shipping RNA with Dry ice
1) RNA stability is greatly depends upon
Temperature, Presence of RNase, Processing time (for freezing and thawing)
2) Delivery options
Option 1 : Deliver purified (Frozen) RNA with dry ice
Option 2 : using RNA stabilizing reagents (commercially available)
3) Basic Rule of Thumb
① Generally, EtOH precipitated RNA shows greater stability.
② Sample should be delivered within 96 hours
③ Either shipping EtOH precipitated sample or using ~8lb (3.6 kg) / 24 hours of dry ice to ensure the cold temperature
※ For this illustration, 16 lb (7.2 kg) of dry ice are used
Samples, TotalOmics RNA sample sheet, moderate amount of dry ice, insulator (newspaper), plastic bags, zipper bags and a box tape
2. TotalOmics RNA Sample Sheet
Step 1 : Purify total RNA
Step 2 : Perform QC
Step 3 : Fill the QC data in TotalOmics Sample sheet
Step 4 : Fill in RIN Score if Bio-Analyzer is available
Step 5 : Freeze and store at -80°C until shipping
Available at : Downloading Documents Page
3. Labeling Samples
Labeling is extremely important for accurate downstream analysis.
The best labeling example for shipping condition
Good for shipping condition, but still possible to fall off due to surface condensation
We recommend you to label the tube again.
4. Packing Samples
Wrap the tube with para-film to prevent samples from evaporation zipper bag
Enclose sample sheet and labeled sample tubes into the zipper bag
Pack the samples as the picture above
※ Box wall should be at least 1.5 inch (38mm) thick
Cover the samples completely with remaining half of dry ice
Seal the plastic bag (not too tight – plastic bag might explode especially in summer)
Fill the gap between the plastic bag and box with insulator (e.g. newspaper)
Theragen Etex Bio Institute Co., Ltd
(443-270) 4F, A-dong, AICT bldg, 145 Gwanggyo-ro, Yeongtong-gu, Suwon-si, Gyeonggi-do, Republic of Korea
Overseas Contact Point
Direct: +82 31 888 9313 (GMT +9)
Home page : http://www.theragenetex.com
Lui-dong, Yeongtong-gu Suwon-si Gyeonggi-do Korea
E : firstname.lastname@example.org
P : 82-31-888-9328
4th fl. AICT bldg. A, 145 Gwanggyo-ro, Yeongtong-gu, Suwon-si, Gyeonggi-do, Republic of Korea, 16229
Phone +82-31-888-9313 / Fax +82-31-888-9335